Lamellar keratoplasty was done to six eyes with preserved rabbit corneas including scleral rim of 1-2mm which were preserved in three kinds of 5cc- alcohol-glycerine (A. G) solution at room temperature for the period of one month to three years and their macroscopical and histological study was carried out.
Three kinds of solution were;
1. A. G. No. 1 solution; 50 % ethyl alcohol and 100 % glycerine mixed by equal ratio.
2. A. G. No. 2 solution; 50% ethyl alcohol and 100 glycerine mixed by two to one ratio. 3. A. G. No. 3 solution; 507. ethyl alcohol and 100 % glycerine mixed by three to one ratio. The results of the above mentioned lamellar keratoplasty with the corneas preserved in three solutions were uniformly good in all groups.
The results of keratoplasty with the corenas preserved for 3 years were as follows;
1. Transplantation of the preserved corneas in A. G. No. 1 solution showed transparent adhesions in two eyes, partial opacity in one, total opacity in two, and rejection in one. Neo-vascularization in this group was more prominent than in other groups.
2. Transplantation of the corneas preserved in A. G. No. 2 solution resulted in transparent adhesion in four eyes, partial pacity one, and total opacity in one and this result could be evaluated as the best of all three groups.
3. Transplantation of the corneas preserved in A. G. No. 3 solution showed transparent adhesion in two eyes, partial opacity in three, and total opacity in one and this result was slightly inferior to that of the second group.
4. Lamellar keratoplasty with human cornea preserved in A. G. No. 2 solution for 18 and 33 months in the 2 cases of corneal leucoma after recurrent meta-herpetic keratitis, resulted in success respectively.
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